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p sting  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc p sting
    P Sting, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 340 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/72971s/Phospho-STING+(Ser365)+Rabbit+mAb/pmc12813888-103-23-25
    Average 97 stars, based on 340 article reviews
    p sting - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Incubation:

    Article Title: STING agonism enhances anti-tumor immune responses and therapeutic efficacy of PARP inhibition in BRCA -associated breast cancer
    Article Snippet: .. Membranes were blocked in 5% milk/TBS-T (Boston BioProducts) and incubated with the following primary antibodies: phospho-TBK1/NAK (Ser172) (D52C2) XP Rabbit mAb (1:500) [Cell Signaling Technology (CST) #5483S] (for human cells), TBK1 (S172) (1:1000) (Abgent #AP7887a-ev) (for murine cells), TBK1/NAK (D1B4) Rabbit mAb (1:1000) (CST #3504S), phospho-STING (Ser366) (D7C3S) Rabbit mAb (1:1000) (CST #19781S) (for human cells), phospho-STING (Ser365) (D8F4W) Rabbit mAb (1:1000) (CST #72971S) (for murine cells), STING/TMEM173 (1:1000) (Novus Biologicals #NBP224683), Vinculin (1:1000) (CST #4650S). .. Immunodetection was performed using SuperSignal West Pico and Femto Chemiluminescent Substrate (Thermo Fisher Scientific).

    Article Title: STING agonism enhances anti-tumor immune responses and therapeutic efficacy of PARP inhibition in BRCA-associated breast cancer.
    Article Snippet: .. Membranes were blocked in 5% milk/TBS-T (Boston BioProducts) and incubated with the following primary antibodies: phospho-TBK1/NAK (Ser172) (D52C2) XP Rabbit mAb (1:500) [Cell Signaling Technology (CST) #5483S] (for human cells), TBK1 (S172) (1:1000) (Abgent #AP7887a-ev) (for murine cells), TBK1/NAK (D1B4) Rabbit mAb (1:1000) (CST #3504S), phospho-STING (Ser366) (D7C3S) Rabbit mAb (1:1000) (CST #19781S) (for human cells), phospho-STING (Ser365) (D8F4W) Rabbit mAb (1:1000) (CST #72971S) (for murine cells), STING/TMEM173 (1:1000) (Novus Biologicals #NBP224683), Vinculin (1:1000) (CST #4650S). .. Immunodetection was performed using SuperSignal West Pico and Femto Chemiluminescent Substrate (Thermo Fisher Scientific).

    other:

    Article Title: PCBP1 binding to single-stranded poly-cytosine motifs enhances cGAS sensing and impairs breast cancer development
    Article Snippet: Anti-mouse phospho-STING , Cell Signaling Technology , cat. no. 72971S.

    Article Title: Saturated fatty acids dampen the immunogenicity of cancer by suppressing STING.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Palmitic acid, R99% Sigma-Aldrich Cat# P0500 Palmitoleic acids, R98.5% Sigma-Aldrich Cat# P9417 Myristic acids, R99% Sigma-Aldrich Cat# M3128 Sodium oleate, R99% Sigma-Aldrich Cat# O7501-1G Sodium hydroxide 1.0 N Solution Sigma-Aldrich Cat# S2770-100ML Tris-HCl pH 8.0 ThermoFisher Cat# BP1758500 Triton X-100 Sigma-Aldrich Cat# X100 Sodium dodecyl sulfate Sigma-Aldrich Cat# 74255-250G Sodium chloride Sigma-Aldrich Cat# S3014-5KG Sodium deoxycholate Sigma-Aldrich Cat# D6750 Sodium fluoride Sigma-Aldrich Cat# 201154 Mini Protease Inhibitor Cocktail Roche Cat# 11836170001 HaltTM Phosphatase Inhibitor Cocktail Thermo-Fisher Cat# 78420 Retrieval buffer Biogenex Cat# Hk081-20k Harris Hematoxylin Fisher Scientific Cat# NC9520196 Isopropanol Fisher Scientific Cat# A426P-4 Oil Red O – Isopropanol Solutio Rowley Biochemical Cat# H-503-1B 3,30-Diaminobenzidine tablets (DAB) Sigma Aldrich Cat# D4168-5SET alamarBlue Cell Viability Reagent Invitrogen Cat# DAL1025 ACK lysing buffer Gibco Cat# A1049201 Ficoll-Paque PLUS density gradient media GE Healthcare Cat# 17-1440-03 Critical commercial assays RNeasy Plus Mini Kit Qiagen Cat# 74134 QIAshredder Qiagen Cat# 79654 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat# 4368814 RNAse inhibitor Applied Biosystems Cat# 8080119 LumiKineTM Xpress mIFN-b 2.0 kit Invivogen Cat# luex-mifnbv2 Tnf-a Mouse ELISA kit Invivogen Cat# BMS6073 CellTraceTM CFSE Cell Proliferation Kit Invitrogen Cat# C34554 EasySep Dead Cell Removal (Annexin V) Kit STEMCELL Technologies Cat# 17899 Anti-HA Agarose Thermo Fisher Scientific Cat# 26181 anti-FLAG M2 Affinity gel Millipore Sigma Cat# A2220 VECTASTAIN ABC Kits (HRP) Vectastain Cat# PK-4001 Cholesterol/Cholesterol Ester-GloTM Assay Promega Cat# J3190 Murine EasySep CD8+ T cell isolation kit Stemcell Technologies Cat# 19853 Dynabeads CD3/CD28 Gibco Cat# 11456D Zombie AquaTM Fixable Viability Kit BioLegend Cat# 423101 Deposited data Single-cell RNA-Seq dataset NCBI Sequence Read Archive PRJNA756027 Experimental models: Cell lines PCI-13 University of Pittsburgh RRID:CVCL_C182 FaDu ATTC RRID:CVCL_1218 UMSCC22A University of Michigan RRID:CVCL_7731 MOC2-E6/7 Harvard University RRID:CVCL_ZD33 Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratory RRID:IMSR_JAX:000664 Mouse: B6.129S7-Rag1tm1Mom/J Jackson Laboratory RRID: IMSR_JAX:002216 Mouse: B6.129S2-Ifnar1tm1Agt/Mmjax Jackson Laboratory RRID:IMSR_JAX:002216 (Continued on next page) 20 Cell Reports 42, 112303, April 25, 2023

    Immunofluorescence:

    Article Title:
    Article Snippet: .. 1) Anti-mouse phospho-TBK1/NAK (Ser172) (D52C2), Cell Signaling Technology, cat. no. 5483S, 1: 1000 dilution; 2) Anti-mouse TBK1/NAK (D1B4), Cell Signaling Technology, cat. no. 3504T, 1: 1000 dilution; 3) Anti-mouse phospho-IRF3 (Ser396) (4D4G), Cell Signaling Technology, cat. no. 4947S, 1: 1000 dilution; 4) Anti-mouse IRF-3 (D83B9), Cell Signaling Technology, cat. no. 4302S, 1: 1000 dilution; 5) Anti-mouse phospho-STING (Ser365) (D8F4W), Cell Signaling Technology, cat. no. 72971S, 1: 1000 dilution; 6) Anti-mouse STING/TMEM173, ABclonal Tech, cat. no. A21051, 1: 10000 dilution; 7) Anti-mouse Tubulin ", Bioss, cat. no. bs-20694R, 1: 1000 dilution; 8) Anti-rabbit IgG, HRP-linked Antibody, Cell Signaling Technology, cat. no. 7074S, 1: 2000 dilution; The following antibodies were used for immunofluorescence staining. ..

    Staining:

    Article Title:
    Article Snippet: .. 1) Anti-mouse phospho-TBK1/NAK (Ser172) (D52C2), Cell Signaling Technology, cat. no. 5483S, 1: 1000 dilution; 2) Anti-mouse TBK1/NAK (D1B4), Cell Signaling Technology, cat. no. 3504T, 1: 1000 dilution; 3) Anti-mouse phospho-IRF3 (Ser396) (4D4G), Cell Signaling Technology, cat. no. 4947S, 1: 1000 dilution; 4) Anti-mouse IRF-3 (D83B9), Cell Signaling Technology, cat. no. 4302S, 1: 1000 dilution; 5) Anti-mouse phospho-STING (Ser365) (D8F4W), Cell Signaling Technology, cat. no. 72971S, 1: 1000 dilution; 6) Anti-mouse STING/TMEM173, ABclonal Tech, cat. no. A21051, 1: 10000 dilution; 7) Anti-mouse Tubulin ", Bioss, cat. no. bs-20694R, 1: 1000 dilution; 8) Anti-rabbit IgG, HRP-linked Antibody, Cell Signaling Technology, cat. no. 7074S, 1: 2000 dilution; The following antibodies were used for immunofluorescence staining. ..



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